ABSTRACT
Objective: To determine the metabolic response associate with dengue infection based on human gender metabolic differences by means of 1H NMR-spectrometry. Methods: The mid-stream urine collected from both male and female patients diagnosed with dengue fever at Penang General Hospital and fourty-three healthy individuals were analyzed with 1H NMR spectroscopy, followed by chemometric multivariate analysis. NMR signals which highlighted in the OPLS-DA S-plot were further selected and identified using Human Metabolome Database, Chenomx Profiler. Results: The results pointed out that NMR urine profiling was able to capture human gender metabolic differences that are important for the distinction of classes of individuals of similar physiological conditions; infected with dengue. Distinct differences between dengue infected patients versus healthy individuals and subtle differences in male versus female infected with dengue were found to be related to the metabolism of amino acid and tricarboxylic acid intermediates cycle. Conclusions: The 1H NMR metabolomic investigation combined with appropriate algorithms and pattern recognition procedures, gave an evidence for the existence of distinct metabolic differentiation of individuals, according to their gender, modulates with the infection risk.
ABSTRACT
OBJECTIVE@#To determine the metabolic response associate with dengue infection based on human gender metabolic differences by means of (1)H NMR-spectrometry.@*METHODS@#The mid-stream urine collected from both male and female patients diagnosed with dengue fever at Penang General Hospital and fourty-three healthy individuals were analyzed with (1)H NMR spectroscopy, followed by chemometric multivariate analysis. NMR signals which highlighted in the OPLS-DA S-plot were further selected and identified using Human Metabolome Database, Chenomx Profiler.@*RESULTS@#The results pointed out that NMR urine profiling was able to capture human gender metabolic differences that are important for the distinction of classes of individuals of similar physiological conditions; infected with dengue. Distinct differences between dengue infected patients versus healthy individuals and subtle differences in male versus female infected with dengue were found to be related to the metabolism of amino acid and tricarboxylic acid intermediates cycle.@*CONCLUSIONS@#The (1)H NMR metabolomic investigation combined with appropriate algorithms and pattern recognition procedures, gave an evidence for the existence of distinct metabolic differentiation of individuals, according to their gender, modulates with the infection risk.
ABSTRACT
In this study ,upon exposure of laboratory Egyptian B. alexandrina and B. glabrata snails to its homologus strain of S. mansoni, two different phenotype strains " susceptible and resistant " were distinguished . The aim of this study is to investigate the biological differences between the two types of Biomphalaria snails either susceptible or resistant at different ages. The investigated parameters included mortality, survival, percentage of susceptibility / resistance to parasitic infection and fecundity. The results of the preliminary exposure experiment revealed that parental snail stock was not completely susceptible and tend to be partially refractory to infection. The results also showed a strong association between the levels of egg production and susceptibility to parasite infection. Where breeding experiment revealed that, total egg number, E/M and EM values produced by resistant phenotype of both snail species were more than those produced by susceptible progeny snails. Also, it was noticed that, egg production is associated inversely proportion with snail age